Mine would have to be the moment in Round 6 when I realized -
I was GC of a ViruS gal in a cluster of 3 ViruS and about 12 FoS/XeTa gals (cluster 7, didn't become ViruS myself til halfway thru)
And that the GC of the bigger ViruS gal in the cluster and I simply could not work together, as the personalities of our respective gals were too different . (we played for fun, they played solely for rank)
It was first and only time I had to face the fact that I had no allies in my cluster, and many enemies.